Cloning and functional expression of poly(ADP-ribose) polymerase cDNA from Sarcophaga peregrina
نویسندگان
چکیده
منابع مشابه
Cloning and expression of cDNA for human poly(ADP-ribose) polymerase.
cDNAs encoding poly(ADP-ribose) polymerase from a human hepatoma lambda gt11 cDNA library were isolated by immunological screening. One insert of 1.3 kilobases (kb) consistently hybridized on RNA gel blots to an mRNA species of 3.6-3.7 kb, which is consistent with the size of RNA necessary to code for the polymerase protein (116 kDa). This insert was subsequently used in both in vitro hybrid se...
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objective: the aim of present study was cloning and expression of phic31 integrase cdna in a bacterial expression vector. thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. materials and methods: in this experimental study, phic31 cdna was subcloned into a prokaryotic expression vector and transformed into e.coli bl21 (de3). recombinant phic31 in...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1994
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1994.tb18662.x